OMX

Super-resolution Microscopy in the Department of Cell Biology at Harvard Medical School


Access

The OMX Blaze was acquired using funds from the Department of Cell Biology, the Office of Tools and Technology, and the Laboratory of Systems Pharmacology.  It is available for use by labs whose Principle Investigator has a primary appointment in one of the HMS basic science departments and LSP.

Contact Us for Access

OMX Blaze Capabilities

  • Twofold increase in lateral (120nm) and axial (340nm) resolution with 3D Structured illumination
  • Fast 3D-SIM: 1 μm Z-stack (120 images) in as fast as 1 frame per second (at 1ms exposure)

In addition to SIM, the OMX Blaze also offers

  • Ring TIRF, greatly reducing interference fringing
  • FRAP / Photoactivation / Photoconversion
  • Simultaneous 3-color widefield imaging with three sCMOS cameras
  • Hardware focus maintenance
SIM vs Widefield

170nm fluorescent beads imaged with widefield (WF) and structured illumination (SIM)


OMX Blaze Capabilities

For more detailed information, view the OMX Blaze technical specifications

For important sample prep information, see our sample prep guidelines

SEE IMAGES FROM THE OMX

The OMX Team

Contact us

The OMX is open to labs whose Principle Investigator has a primary appointment in one of the HMS basic science departments.

For questions about the OMX, please email Talley Lambert

If you want to be trained on the OMX or if you want to come in for a free consultation to discuss whether the OMX might benefit your experiment, please fill out a training request